IL12 Protein

Catalog No. ABIN6700020

Supplier Product No.: 010-001-v51-0010

Recombinant IL12 protein expressed in Escherichia coli (E. coli).

Quick Overview for IL12 Protein (ABIN6700020)

Target: IL12 (Interleukin 12 (IL12))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 95,00%

Product Details

Supplier Product No.: 010-001-v51-0010

Supplier: Rockland

Purpose: Mouse Interleukin-12 Recombinant Protein

Purification: Interleukin-12 purity was determined to be greater than 95% as determined by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the induced expression of CD25 on CD8+ mouse T cells and is typically detectable starting at 0.1 ng/mL.

Application Details

Application Notes:

Application Note: Interleukin-12 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-12 in immunological assays. Lyophilized in 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Buffer: Lyophilized in 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: Il12a/Il12b

Background:

Synonyms: NKSF, CLMF

Background: Interleukin-12 (IL-12) is an important mediator of cellular-immunity produced by dendritic cells, macrophages and B cells when stimulated with antigen. IL-12 is involved in differentiating precursor helper T cells to Th1 and/or Th2 subtypes and promotes the production of IFN gamma and TNF alpha. Mouse IL-12 is active on human and mouse cells. Recombinant mouse IL-12 is a glycosylated, disulfide-linked heterodimer. It is containing one p35 su

UniProt: P43432

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome