IL-7 Protein

Catalog No. ABIN6700167

Supplier Product No.: 009-001-b94

Recombinant IL-7 protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-7 Protein (ABIN6700167)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 98,00%

Product Details

Supplier Product No.: 009-001-b94

Supplier: Rockland

Purpose: IL-7 Human Recombinant Protein

Purification: Purity was determined to be greater than 98% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE, against known standard.

Endotoxin Level: Measured by LAL is 0.01 EU/μg protein.

Biological Activity Comment: Recombinant H-IL-7 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of mouse 2E8 cells is less then 0.5ng/ml.

Application Details

Application Notes:

Application Note: IL-7 protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-7 in immunological assays. Buffer Formulation: 10 mM Acetic Acid.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Concentration: 0.1 mg/mL

Buffer: Buffer Formulation: 10 mM Acetic Acid.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Alternative Name: IL7

Background:

Synonyms: Interleukin 7 cytokine, Interleukin-7, IL-7

Background: Interleukin 7 (IL-7), which is constitutively produced particularly by stromal cells from the bone marrow and thymus, plays a crucial role in T cell homeostasis. This cytokine is implicated in thymopoiesis because it sustains thymocyte proliferation and survival. It regulates peripheral naive T cell survival by modulating the expression of the anti-apoptotic molecule Bcl-2, and sustains peripheral T cell expansion in response to antigenic stimulation. Infection by the human immunodeficiency virus (HIV) leads to severe T lymphopenia and general immune dysfunction. Increased IL-7 plasma levels are generally observed in HIV-infected patients. IL-7 may also support bioenergetics that control cell fate decisions in lymphocytes. Recombinant Human IL-7 produced in E. coli is a single, non-glycosylated polypeptide chain containing 153 amino acids and having a molecular mass of 17,412 Daltons.

UniProt: D0EYG7

Pathways: JAK-STAT Signaling