IL-7 Protein

Catalog No. ABIN6700169

Supplier Product No.: 010-001-b94-0020

This Recombinant IL-7 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL-7 Protein (ABIN6700169)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 98,00%

Product Details

Supplier Product No.: 010-001-b94-0020

Supplier: Rockland

Purpose: Mouse Interleukin-7 Recombinant Protein

Purification: Interleukin-7 purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by dose-dependent induction of 2E8 cell proliferation and is typically less than 0.2 ng/mL.

Application Details

Application Notes:

Application Note: Interleukin-7 Recombinant Protein is suitable as a control for polyclonal or monoclonal anti-Interleukin-7 in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Alternative Name: Il7

Background:

Synonyms: Lymphopoietin 1 (LP-1), pre-B cell Factor

Background: Interleukin 7 (IL-7) is a hematopoietic cytokine that is constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cell s, and natural killer cells. Human and mouse IL-7 show species cross-reactivity. Recombinant mouse IL-7 is a non-glycosylated protein, containing 172 amino acids, with a molecular weight of 17.4 kDa.

UniProt: P10168

Pathways: JAK-STAT Signaling