STK31 Protein (GST tag)

Catalog No. ABIN6700497

Supplier Product No.: 009-001-t62s

Recombinant STK31 protein expressed in Insect cells (Sf9).

Quick Overview for STK31 Protein (GST tag) (ABIN6700497)

Target: STK31 (serine/threonine Kinase 31 (STK31))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Western Blotting (WB)

Purity: >80%

Product Details

Purification tag / Conjugate: This STK31 protein is labelled with GST tag.

Supplier Product No.: 009-001-t62s

Supplier: Rockland

Purpose: STK31 recombinant protein-GST fusion protein

Purification: Recombinant human STK31 (496-end) was expressed by baculovirus in Sf9 insect cell using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >80% by densitometry.

Application Details

Application Notes:

Application Note: STK31 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. STK31 Protein is suitable for use in Western Blot. Expect a band approximately ~86 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: STK31 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: STK31 (serine/threonine Kinase 31 (STK31))

Alternative Name: STK31

Background:

Synonyms: SGK396, TDRD8, Serine/threonine-protein kinase 31, Serine/threonine-protein kinase NYD-SPK, Sugen kinase 396, SgK396, STK31

Background: STK31 is a serine/threonine protein kinase that contains a tudor domain found in RNA-interacting proteins, and a coiled-coil domain. Tudor domains are found in many eukaryotic organisms and have been implicated in protein-protein interactions in which methylated protein substrates bind to these domains. The STK31 gene is conserved across many species and was originally identified as a gene expressed in mouse spermatogonia but not in somatic tissues (1). STK31 has been shown to be specifically expressed in the human testis. STK31 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Cardiovascular Disease, ERK/MAPK Pathway, Neurobiology, PKA/PKC Pathway, and Ser/Thr Kinases research.

NCBI Accession: NM_031414