MAPT Protein

Catalog No. ABIN6700607

Supplier Product No.: 009-001-t72s

This Recombinant MAPT protein is expressed in Escherichia coli (E. coli).

Quick Overview for MAPT Protein (ABIN6700607)

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Purity: >90%

Product Details

Supplier Product No.: 009-001-t72s

Supplier: Rockland

Purpose: Tau-316 recombinant protein

Purification: Recombinant full-length human tag-free Tau-316 was expressed in E. coli cells. The purity was determined to be >90% by densitometry.

Application Details

Application Notes:

Application Note: Tau-316 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. Tau-316 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~39 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Other: Kinase Assay-User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: Tau-316 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Alternative Name: MAPT

Background:

Synonyms: Fetal-tau, Tau-A, TAU, MAPT, MSTD, PPND, DDPAC, MAPTL, MTBT1, MTBT2, FTDP-17, Microtubule-associated protein tau

Background: Tau-316 encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. Tau-316 transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type (1). Tau-316 gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy (2). Tau-316 Protein is ideal for investigators involved in Signaling Proteins, Tau Proteins, Invasion/Metastasis, Neurobiology, and p38 Pathway research.

UniProt: P10636-3

Pathways: MAPK Signaling, Microtubule Dynamics, M Phase, Regulation of Cell Size