ZEBOV GP Protein (AA 33-501) (His tag)

Catalog No. ABIN6941974

Product Details

Target: ZEBOV GP (Zaire Ebola Virus Envelope Glycoprotein (ZEBOV GP))

Protein Type: Recombinant

Protein Characteristics: AA 33-501

Origin: Ebola Virus

Source: HEK-293 Cells

Purification tag / Conjugate: This ZEBOV GP protein is labelled with His tag.

Purpose: Recombinant Ebolavirus EBOV (subtype Zaire,strain Kikwit-95) Envelope Glycoprotein 1 (GP1) protein produced in HEK293 cells. Protein contains a C-terminal 6x His-tag

Specificity: Ebola virus envelope glycoprotein is initially produced as a precursor which is then cleaved into two subunits, GP1 and GP2. This is a recombinant version of GP1, produced in HEK293 cells, and incorporating a C-terminal 6x His-tag with greater than 95% purity.

Characteristics: Ebola Virus Envelope Glycoprotein (GP1) (Zaire)

Purity: >95 %

Application Details

Comment:

This Ebola virus envelope glycoprotein (GP1) is derived from the GP1 sequence (Accession # AAQ55048.1), expressing Ile33 – Arg501. It is fused with a polyhistidine tag at the C-terminus and has a calculated MW of 51.6kDa. The predicted N-terminus is Ile 33. The protein is expressed in HEK293 cells. DTT-reduced protein migrates as 120-140kDa in SDS-PAGE.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS pH 7.4

Storage: 4 °C

Storage Comment: 4°C

Target Details

Target: ZEBOV GP (Zaire Ebola Virus Envelope Glycoprotein (ZEBOV GP))

Alternative Name: Ebola Envelope Glycoprotein (GP1) (Zaire) (ZEBOV GP Products)

Target Type: Viral Protein

Background: Ebola virus envelope glycoprotein is initially produced as a precursor known as pre-GP, which is cleaved by furin into two subunits, GP1 and GP2, which remain associated through a disulfide linkage between Cys53 of GP1 and Cys609 of GP2. This heterodimer assembles into a 450-kDa trimer at the surface of nascent virions.
The virion-attached GP is critical in the EBOV life cycle, as it is solely responsible for attachment, fusion and entry of target cells. Moreover, GP is responsible for critical pathogenic differences among viral species.

UniProt: AAQ55048.1