SARS-CoV-2 Spike S1 Protein (His tag)

Catalog No. ABIN6952318

Product Details

Target: SARS-CoV-2 Spike S1

Protein Type: Recombinant

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purification tag / Conjugate: This SARS-CoV-2 Spike S1 protein is labelled with His tag.

Purpose: Recombinant SARS-CoV-2 S1 Subunit Protein with C-terminal His-tag, secreted from the serum-free medium of transfected HEK293 cells.Purchase will also include one vial of normal control, the culture supernatant of HEK293 cell transfected with empty expression vector.

Characteristics: Expressed Region:Val16 - Gln690
C-terminal histidine tag

Purification: Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80 °C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Cell culture supernatant of transfected HEK293 cells, serum-free.

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment:

Storage: -20°C

storageComment: Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Target Details

Target: SARS-CoV-2 Spike S1

Abstract: SARS-CoV-2 Spike S1 Products

Target Type: Viral Protein

Molecular Weight: 75 kDa

Gene ID: 43740568

UniProt: P0DTC2