SARS-CoV-2 NSP12 (RdRP) Protein (His tag)
Crystallography grade
SARS-CoV-2 RdRP
Origin: SARS Coronavirus-2 (SARS-CoV-2)
Host: Insect Cells
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Crys, ELISA, SDS, WB
1 image
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- Target See all SARS-CoV-2 NSP12 (RdRP) (SARS-CoV-2 RdRP) products
- SARS-CoV-2 NSP12 (RdRP) (SARS-CoV-2 RdRP) (SARS-CoV-2 RNA-dependent RNA Polymerase (NSP12) (SARS-CoV-2 RdRP))
- Protein Type
- Recombinant
- Origin
- SARS Coronavirus-2 (SARS-CoV-2)
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Source
- Insect Cells
- Purification tag / Conjugate
- This SARS-CoV-2 NSP12 (RdRP) protein is labelled with His tag.
- Application
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
- MSADAQSFLNR VCGVSAARLT PCGTGTSTDV VYRAFDIYND KVAGFAKFLK TNCCRFQEKD EDDNLIDSYF VVKRHTFSNY QHEETIYNLL KDCPAVAKHD FFKFRIDGDM VPHISRQRLT KYTMADLVYA LRHFDEGNCD TLKEILVTYN CCDDDYFNKK DWYDFVENPD ILRVYANLGE RVRQALLKTV QFCDAMRNAG IVGVLTLDNQ DLNGNWYDFG DFIQTTPGSG VPVVDSYYSL LMPILTLTRA LTAESHVDTD LTKPYIKWDL LKYDFTEERL KLFDRYFKYW DQTYHPNCVN CLDDRCILHC ANFNVLFSTV FPPTSFGPLV RKIFVDGVPF VVSTGYHFRE LGVVHNQDVN LHSSRLSFKE LLVYAADPAM HAASGNLLLD KRTTCFSVAA LTNNVAFQTV KPGNFNKDFY DFAVSKGFFK EGSSVELKHF FFAQDGNAAI SDYDYYRYNL PTMCDIRQLL FVVEVVDKYF DCYDGGCINA NQVIVNNLDK SAGFPFNKWG KARLYYDSMS YEDQDALFAY TKRNVIPTIT QMNLKYAISA KNRARTVAGV SICSTMTNRQ FHQKLLKSIA ATRGATVVIG TSKFYGGWHN MLKTVYSDVE NPHLMGWDYP KCDRAMPNML RIMASLVLAR KHTTCCSLSH RFYRLANECA QVLSEMVMCG GSLYVKPGGT SSGDATTAYA NSVFNICQAV TANVNALLST DGNKIADKYV RNLQHRLYEC LYRNRDVDTD FVNEFYAYLR KHFSMMILSD DAVVCFNSTY ASQGLVASIK NFKSVLYYQN NVFMSEAKCW TETDLTKGPH EFCSQHTMLV KQGDDYVYLP YPDPSRILGA GCFVDDIVKT DGTLMIERFV SLAIDAYPLT KHPNQEYADV FHLYLQYIRK LHDELTGHML DMYSVMLTND NTSRYWEPEF YEAMYTPHTV LQHHHHHH
- Characteristics
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The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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C-Terminal His-Tag
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 50 mM Hepes, pH 8; 1000 mM NaCl; 1 % Glycerin, 3 mM MgCl2 and protease inhibitors
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- SARS-CoV-2 NSP12 (RdRP) (SARS-CoV-2 RdRP) (SARS-CoV-2 RNA-dependent RNA Polymerase (NSP12) (SARS-CoV-2 RdRP))
- Alternative Name
- SARS-CoV-2 RNA-directed RNA Polymerase (SARS-CoV-2 RdRP Products)
- Synonyms
- pp1ab Protein, ORF1a polyprotein;ORF1ab polyprotein Protein, ORF1ab Protein
- Target Type
- Viral Protein
- Background
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Cleavage product of rpp1ab (AA 5393-5324) polyprotein from the Wuhan-Hu-1 isolate, Wuhan, China Dec 2019
Responsible for replication and transcription of the viral RNA genomeB - Molecular Weight
- 106661
- NCBI Accession
- YP_009725307
- UniProt
- P0DTD1
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