SARS-CoV-2 Spike S1 Protein (RBD) (mFc Tag)

Catalog No. ABIN6952742

Recombinant SARS-CoV-2 Spike S1 protein expressed in HEK-293 Cells.

Quick Overview for SARS-CoV-2 Spike S1 Protein (RBD) (mFc Tag) (ABIN6952742)

Target: SARS-CoV-2 Spike S1

Protein Type: Recombinant

Biological Activity: Active

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Application: SDS-PAGE (SDS), Functional Studies (Func)

Purity: > 95 % by SDS-PAGE.

Product Details

Protein Characteristics: RBD

Purification tag / Conjugate: This SARS-CoV-2 Spike S1 protein is labelled with mFc Tag.

Sequence: RVQPTESIVR FPNITNLCPF GEVFNATRFA SVYAWNRKRI SNCVADYSVL YNSASFSTFK CYGVSPTKLN DLCFTNVYAD SFVIRGDEVR QIAPGQTGKI ADYNYKLPDD FTGCVIAWNS NNLDSKVGGN YNYLYRLFRK SNLKPFERDI STEIYQAGST PCNGVEGFNC YFPLQSYGFQ PTNGVGYQPY RVVVLSFELL HAPATVCGPK KSTNLVKNKC VNF

Specificity: Arg319-Phe541

Characteristics: Recombinant 2019-nCoV Spike RBD Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of 2019-ncov Spike RBD (Accession #YP_009724390.1) fused with a mFc tag at the C-terminus.

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 Protein at 2 μg/mL (100 μL/well) can bind Recombinant SARS-COV-2 Spike RBD-mFc Protein, the EC₅₀ of Recombinant SARS-COV-2 Spike RBD-mFc Protein is 2.58 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.|or This product is stable at ≤ -70°C for up to 6 months from the date of receipt. |For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.

Target Details

Target: SARS-CoV-2 Spike S1

Target Type: Viral Protein

Background: Envelope,SARS-CoV-2 Spike RBD (N501Y),Spike,Spike ECD,Spike RBD,Spike S1,Spike S2,Spike S2 ECD,S1-RBD protein,NCP-CoV RBD Protein,novel coronavirus RBD Protein,2019-nCoV RBD Protein,S glycoprotein Subunit1 RBD Protein

Gene ID: 43740568

UniProt: P0DTC2