SARS-CoV-2 Spike Protein (His-Avi Tag)
Quick Overview for SARS-CoV-2 Spike Protein (His-Avi Tag) (ABIN6953018)
Target
See all SARS-CoV-2 Spike ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purification tag / Conjugate
- This SARS-CoV-2 Spike protein is labelled with His-Avi Tag.
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Characteristics
- DNA sequence encoding the COVID-19 Spike S protein extracellular domain,[16-1188] R683A (accession # QHD43416.1) in 3 replicates including a C-terminal His AVI tag was expressed in HEK Cells.
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Endotoxin Level
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
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Biological Activity Comment
- The activity was tested by binding Human ACE2 in functional ELISA assay
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Comment
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Recombinant 2019-nCoV Spike S trimer protein is a protein consisting of the S1+S2 subunits amino acid residues. The protein has a theoretical MW of 140kDa,however due to glycosylation migrates as an approximately 200 kDa protein on reduced SDS-PAGE.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
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Buffer
- Recombinant nCoV-2019 Spike S trimer protein was lyophilized from 0.2 μm filtered PBS pH 7.4.
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Storage
- -20 °C
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Storage Comment
- The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
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- SARS-CoV-2 Spike
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Target Type
- Viral Protein
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Molecular Weight
- 140 kDa
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Gene ID
- 43740568
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UniProt
- P0DTC2
Target
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