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SARS-CoV-2 Spike Protein (His-Avi Tag)

This Recombinant SARS-CoV-2 Spike protein is produced in HEK-293 Cells.
Catalog No. ABIN6953018

Quick Overview for SARS-CoV-2 Spike Protein (His-Avi Tag) (ABIN6953018)

Target

See all SARS-CoV-2 Spike Proteins
SARS-CoV-2 Spike

Protein Type

Recombinant

Biological Activity

Active

Origin

  • 150
  • 31
  • 7
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  • 3
  • 1
  • 1
SARS Coronavirus-2 (SARS-CoV-2)

Source

  • 136
  • 6
  • 2
  • 2
  • 2
  • 1
  • 1
HEK-293 Cells

Purity

>95 % as determined by SDS-PAGE and HPLC
  • Purification tag / Conjugate

    This SARS-CoV-2 Spike protein is labelled with His-Avi Tag.

    Characteristics

    DNA sequence encoding the COVID-19 Spike S protein extracellular domain,[16-1188] R683A (accession # QHD43416.1) in 3 replicates including a C-terminal His AVI tag was expressed in HEK Cells.

    Endotoxin Level

    Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

    Biological Activity Comment

    The activity was tested by binding Human ACE2 in functional ELISA assay
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  • Comment

    Recombinant 2019-nCoV Spike S trimer protein is a protein consisting of the S1+S2 subunits amino acid residues. The protein has a theoretical MW of 140kDa,however due to glycosylation migrates as an approximately 200 kDa protein on reduced SDS-PAGE.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

    Buffer

    Recombinant nCoV-2019 Spike S trimer protein was lyophilized from 0.2 μm filtered PBS pH 7.4.

    Storage

    -20 °C

    Storage Comment

    The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
  • Target

    SARS-CoV-2 Spike

    Target Type

    Viral Protein

    Molecular Weight

    140 kDa

    Gene ID

    43740568

    UniProt

    P0DTC2
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