SARS-CoV-2 Spike Protein (Fc-Avi Tag)
Quick Overview for SARS-CoV-2 Spike Protein (Fc-Avi Tag) (ABIN6953027)
Target
See all SARS-CoV-2 Spike ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This SARS-CoV-2 Spike protein is labelled with Fc-Avi Tag.
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Characteristics
- DNA sequence encoding the COVID-19 Spike S protein extracellular domain,[14-1211] accession #QHD43416.1 including a C-terminal Fc AVI tag was expressed in HEK Cells. (The furin protease cleavage site was mutated at position 683)
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Endotoxin Level
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
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SARS-CoV-2 Spike (Super Stable Trimer) protein (His tag)
Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
SARS-CoV-2 Spike (Trimer) protein (His-Avi Tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
SARS-CoV-2 Spike (RBD) protein (His-Avi Tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (B.1.1.529 - Omicron), (RBD) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (E484K), (K417N), (N501Y), (RBD) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (B.1.1.7 - alpha), (RBD) protein (Fc Tag)Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha Host: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
SARS-CoV-2 Spike (B.1.617.2 - delta), (RBD) protein (Fc Tag)Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Delta Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
SARS-CoV-2 Spike (RBD) protein (mFc-His Tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining. ELISA, WB
SARS-CoV-2 Spike (Super Stable Trimer) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
SARS-CoV-2 Spike (B.1.640), (RBD) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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Comment
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Recombinant 2019-nCoV Spike S protein is a protein consisting of the S1+S2 subunits with a calculated MW of 160kDa ,due to glycosylation migrates as an approximately 220 kDa protein on SDS-PAGE.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
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Buffer
- Recombinant nCoV-2019 Spike S protein was lyophilized from 0.2 μm filtered PBS pH 7.4
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Storage
- -20 °C
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Storage Comment
- The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
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- SARS-CoV-2 Spike
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Target Type
- Viral Protein
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Gene ID
- 43740568
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UniProt
- P0DTC2
Target
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