SARS-CoV-2 Spike Protein (Super Stable Trimer) (His tag)

Catalog No. ABIN6953299

Recombinant SARS-CoV-2 Spike protein expressed in HEK-293 Cells.

Quick Overview for SARS-CoV-2 Spike Protein (Super Stable Trimer) (His tag) (ABIN6953299)

Target: SARS-CoV-2 Spike

Protein Type: Recombinant

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Application: ELISA

Purity: > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.

Product Details

Protein Characteristics: Super Stable Trimer

Purification tag / Conjugate: This SARS-CoV-2 Spike protein is labelled with His tag.

Purpose: SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS verified)

Sequence: AA 16-1213

Characteristics: SARS-CoV-2 S protein, His Tag, Super stable trimer is the ectodomain of SARS-CoV-2 S protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: This product is specifically designed for serological test development.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Storage: -80 °C

Storage Comment: The product MUST be stored at -70°C or lower upon receipt.

Expiry Date: 3 months

Target Details

Target: SARS-CoV-2 Spike

Target Type: Viral Protein

Background: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molecular Weight: 138.0 kDa