SARS-CoV-2 Spike Protein (Super Stable Trimer) (His tag)

Catalog No. ABIN6953302

Recombinant SARS-CoV-2 Spike protein expressed in HEK-293 Cells and has been mentioned in 2+ publications.

Quick Overview for SARS-CoV-2 Spike Protein (Super Stable Trimer) (His tag) (ABIN6953302)

Target: SARS-CoV-2 Spike

Protein Type: Recombinant

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Application: ELISA

Purity: > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.

Product Details

Protein Characteristics: Super Stable Trimer

Purification tag / Conjugate: This SARS-CoV-2 Spike protein is labelled with His tag.

Purpose: SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS & NS-EM verified)

Sequence: AA 16-1213

Characteristics: SARS-CoV-2 S protein, His Tag, Super stable trimer is the ectodomain of SARS-CoV-2 S protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus.
Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: This product is specifically designed for antibody drug development.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS

Storage: -20 °C,-80 °C

Storage Comment: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state
70°C for 3 months under sterile conditions after reconstitution.

Expiry Date: 12 months

References

Yokoyama, Ichiki: "Nano-size dependence in the adsorption by the SARS-CoV-2 spike protein over gold colloid." in: Colloids and surfaces. A, Physicochemical and engineering aspects, Vol. 615, pp. 126275, (2021) (PubMed).

Wei, Wan, Yan, Wang, Zhang, Yang, Zhang, Fan, Li, Deng, Sun, Gong, Yang, Wang, Wang, Li, Yang, Li, Zhang, Wang, Du, Zong, Yin, Zhang, Wang, Peng, Lin, Feng, Qin, Chen, Gao, Zhang, Cao, Zhong: "HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry." in: Nature metabolism, Vol. 2, Issue 12, pp. 1391-1400, (2020) (PubMed).

Target Details

Target: SARS-CoV-2 Spike

Target Type: Viral Protein

Background: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molecular Weight: 138.0 kDa