2B4 Protein (AA 22-216) (His tag,mFc Tag)

Catalog No. ABIN6961083

This Recombinant 2B4 protein is produced in HEK-293 Cells.

Quick Overview for 2B4 Protein (AA 22-216) (His tag,mFc Tag) (ABIN6961083)

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 22-216

Purification tag / Conjugate: This 2B4 protein is labelled with His tag,mFc Tag.

Purpose: Recombinant human 2B4 protein with C-terminal mouse Fc and 6xHis tag

Specificity: 2B4(Cys22-Ala216) mFc(Pro99-Lys330) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Alternative Name: 2B4

Background: CD244,2B4,SLAMF4,NKR2B4,NAIL,h2B4
This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: predicted molecular mass of 48.8 kDa after removal of the signal peptide. The apparent molecular mass of 2B4-mFc-His is 70-100 kDa due to glycosylation.

Gene ID: 51744

UniProt: Q9BZW8