IL-6 Protein (AA 30-212) (His tag,mFc Tag)

Catalog No. ABIN6961105

This Recombinant IL-6 protein is expressed in HEK-293 Cells.

Quick Overview for IL-6 Protein (AA 30-212) (His tag,mFc Tag) (ABIN6961105)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Immunoassay (IA), Antibody Drug Assay (ADA), Affinity Measurement (AM), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 30-212

Purification tag / Conjugate: This IL-6 protein is labelled with His tag,mFc Tag.

Purpose: Recombinant human IL6 protein with C-terminal mouse Fc and 6xHis tag

Specificity: IL6(Val30-Met212) mFc(Pro99-Lys330) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL6

Background: IL6,Interleukin-6,BSF2,HSF,IFNB2
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants.

Molecular Weight: predicted molecular mass of 47.9 kDa after removal of the signal peptide.

Gene ID: 3569

UniProt: P05231

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome