BTN3A1 Protein (AA 30-254) (His tag,mFc Tag)

Catalog No. ABIN6961119

This Recombinant BTN3A1 protein is produced in HEK-293 Cells.

Quick Overview for BTN3A1 Protein (AA 30-254) (His tag,mFc Tag) (ABIN6961119)

Target: BTN3A1 (Butyrophilin, Subfamily 3, Member A1 (BTN3A1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 30-254

Purification tag / Conjugate: This BTN3A1 protein is labelled with His tag,mFc Tag.

Purpose: Recombinant Human BTN3A1 protein with C-terminal mouse Fc and 6xHis tag

Specificity: BTN3A1(Gln30-Gly254) mFc(Pro99-Lys330) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: BTN3A1 (Butyrophilin, Subfamily 3, Member A1 (BTN3A1))

Alternative Name: BTN3A1

Background: BTN3A1,BTF5,CD277,BTN3.1,BT3.1
The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g., BTN2A1, MIM 613590) and BTN3 (e.g., BNT3A1) genes, which have undergone tandem duplication, resulting in 3 copies of each.

Molecular Weight: predicted molecular mass of 51.36 kDa after removal of the signal peptide.

Gene ID: 11119

UniProt: O00481

Pathways: Activated T Cell Proliferation