SARS-CoV-2 Spike Protein (RBD) (mFc Tag)

Catalog No. ABIN6961175

Recombinant SARS-CoV-2 Spike protein expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for SARS-CoV-2 Spike Protein (RBD) (mFc Tag) (ABIN6961175)

Target: SARS-CoV-2 Spike

Protein Type: Recombinant

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Application: ELISA, Western Blotting (WB)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: RBD

Purification tag / Conjugate: This SARS-CoV-2 Spike protein is labelled with mFc Tag.

Purpose: Recombinant SARS-CoV-2 (2019-nCoV) S protein RBD with C-terminal mouse Fc tag

Specificity: S protein RBD (Arg319-Phe541) mFc (Pro99-Lys330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

References

Wu, Wu, Wang, Liu, Chu, Jiang, Kwong, Chow, Li, Chen: "Microfluidic particle dam for direct visualization of SARS-CoV-2 antibody levels in COVID-19 vaccinees." in: Science advances, Vol. 8, Issue 22, pp. eabn6064, (2022) (PubMed).

Target Details

Target: SARS-CoV-2 Spike

Target Type: Viral Protein

Background: SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.

Molecular Weight: predicted molecular mass of 51.3 kDa after removal of the signal peptide.

Gene ID: 43740568

UniProt: P0DTC2