Zona Pellucida Glycoprotein 3 Protein (ZP3) (Fc Tag)
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- Target See all Zona Pellucida Glycoprotein 3 (ZP3) Proteins
- Zona Pellucida Glycoprotein 3 (ZP3)
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Zona Pellucida Glycoprotein 3 protein is labelled with Fc Tag.
- Purpose
- Recombinant human ZP3 protein with C-terminal human Fc tag
- Specificity
- ZP3 (Gln23-Ser386) hFc (Glu99-Ala330)
- Characteristics
- Extracellular Domain Protein
- Purification
- affinity purification
- Purity
- The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
- Top Product
- Discover our top product ZP3 Protein
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Zona Pellucida Glycoprotein 3 (ZP3) (AA 183-386) protein (His-IF2DI Tag)
ZP3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses ELISA, WB
Zona Pellucida Glycoprotein 3 (ZP3) (AA 1-373) protein (GST tag)ZP3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Zona Pellucida Glycoprotein 3 (ZP3) (AA 23-387) protein (His tag)Crystallography grade ZP3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Zona Pellucida Glycoprotein 3 (ZP3) (AA 23-350) protein (rho-1D4 tag)Crystallography grade ZP3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Zona Pellucida Glycoprotein 3 (ZP3) (AA 23-346), (partial) protein (GST tag)ZP3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute with deionized water
- Buffer
- Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
- Preservative
- Without preservative
- Storage
- -20 °C,-80 °C
- Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- Expiry Date
- 12 months
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- Target
- Zona Pellucida Glycoprotein 3 (ZP3)
- Alternative Name
- ZP3 (ZP3 Products)
- Synonyms
- ZP3A Protein, ZP3B Protein, ZPC Protein, Zp-3 Protein, zp3.2 Protein, Zp-3B Protein, zona pellucida glycoprotein 3 Protein, zona pellucida sperm-binding protein 3 Protein, ZP3 Protein, zp3 Protein, Zp3 Protein
- Background
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Synonymes: OOMD3, Zp-3, ZP3A, ZP3B, ZPC
Description: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus - Molecular Weight
- predicted molecular mass of 66.6 kDa after removal of the signal peptide. The apparent molecular mass of ZP3-hFc is 70-100 kDa due to glycosylation.
- Gene ID
- 7784
- UniProt
- P21754
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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