SARS-CoV-2 Spike Protein (B.1.1.529 - Omicron, NTD) (His tag)

Catalog No. ABIN7041440

This Recombinant SARS-CoV-2 Spike protein is produced in HEK-293 Cells.

Quick Overview for SARS-CoV-2 Spike Protein (B.1.1.529 - Omicron, NTD) (His tag) (ABIN7041440)

Target: SARS-CoV-2 Spike

Protein Type: Recombinant

Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Omicron

Source: HEK-293 Cells

Application: ELISA, SDS-PAGE (SDS)

Purity: > 95% as determined by SDS-PAGE. > 95% as determined by SEC-MALS.

Product Details

Protein Characteristics: B.1.1.529 - Omicron, NTD

Purification tag / Conjugate: This SARS-CoV-2 Spike protein is labelled with His tag.

Purpose: SARS-CoV-2 Spike NTD, His Tag (B.1.1.529/Omicron) (MALS verified)

Characteristics: SARS-CoV-2 Spike NTD, His Tag (B.1.1.529/Omicron) is expressed from human 293 cells (HEK293). It contains AA Ser 13 - Leu 303 (Accession # QHD43416.1(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: B.1.1.529; GISAID clade: GR/484A; Nextstrain clade: 21K). Predicted N-terminus: Ser 13

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C/-80 °C

Storage Comment: For long term storage, the product should be stored at lyophilized state at -20°C or lower.This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 months under sterile conditions after reconstitution.

Expiry Date: 12 monts

Target Details

Target: SARS-CoV-2 Spike

Target Type: Viral Protein

Background: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molecular Weight: 34.6 kDa