Caspase 9 Protein (AA 1-134) (His tag)

Catalog No. ABIN7391390

This Recombinant Caspase 9 protein is expressed in Escherichia coli (E. coli).

Quick Overview for Caspase 9 Protein (AA 1-134) (His tag) (ABIN7391390)

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), SDS-PAGE (SDS), Immunogen (Imm), Positive Control (PC)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-134

Purification tag / Conjugate: This Caspase 9 protein is labelled with His tag.

Purpose: Recombinant Caspase 9 (CASP9)

Sequence: Met1-Gly134

Characteristics: Recombinant Caspase 9 (CASP9), Prokaryotic expression, Met1-Gly134 (Accession # P55211), N-terminal His Tag

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Isoelectric Point: 7.1

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 10 mM PBS ( pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Expiry Date: 12 months

Target Details

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Alternative Name: Caspase 9

Background: APAF3, ICE-LAP6, MCH6, Apoptosis-Related Cysteine Peptidase, Cysteinyl Aspartate Specific Proteinases 9, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6

Molecular Weight: 17kDa

UniProt: P55211

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity