IFNB1 Protein (AA 22-182) (His tag)

Catalog No. ABIN7092862

This Recombinant IFNB1 protein is produced in HEK-293 Cells.

Quick Overview for IFNB1 Protein (AA 22-182) (His tag) (ABIN7092862)

Target: IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Application: Screening Assay (ScA), Immunoassay (IA), Antibody Drug Assay (ADA), Affinity Measurement (AM)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 22-182

Purification tag / Conjugate: This IFNB1 protein is labelled with His tag.

Purpose: Recombinant mouse IFNB1 protein with C-terminal 6xHis tag

Specificity: Mouse IFNB1(Ile22-Asn182) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))

Alternative Name: IFNB1

Background: Ifb,IFN-beta,IFNB
Has antiviral, antibacterial and anticancer activities.[UniProtKB/Swiss-Prot Function]

Molecular Weight: predicted molecular mass of 20.6 kDa after removal of the signal peptide.The apparent molecular mass of mIFNB1-His is 25-35 kDa due to glycosylation.

UniProt: P01575

Pathways: JAK-STAT Signaling, TCR Signaling, TLR Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Autophagy, Inflammasome