Leptin Protein (LEP) (AA 22-167) (His tag)

Catalog No. ABIN7123742

This Recombinant Leptin protein is produced in Escherichia coli (E. coli).

Quick Overview for Leptin Protein (LEP) (AA 22-167) (His tag) (ABIN7123742)

Target: Leptin (LEP)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 95 % by SDS-PAGE gel analyses

Product Details

Protein Characteristics: AA 22-167

Purification tag / Conjugate: This Leptin protein is labelled with His tag.

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/mL in sterile distilled water by gentle pipetting 2-3 times, don't vortex

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % trehalose, pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: -20°C for 12 months as lyophilized, 2-8°C for 1 month under sterile conditions after reconstitution

Expiry Date: 12 months

Target Details

Target: Leptin (LEP)

Alternative Name: leptin

Background: Leptin, Obese protein, Obesity factor, LEP, OB, OBS

Molecular Weight: 16 kDa

UniProt: P41159

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process