Leptin Protein (LEP) (AA 22-171) (His tag)

Catalog No. ABIN7321015

Recombinant Leptin protein expressed in Yeast (Pichia pastoris).

Quick Overview for Leptin Protein (LEP) (AA 22-171) (His tag) (ABIN7321015)

Target: Leptin (LEP)

Protein Type: Recombinant

Origin: Crucian Carp

Source: Yeast (Pichia pastoris)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 22-171

Purification tag / Conjugate: This Leptin protein is labelled with His tag.

Purpose: Recombinant Carassiusauratus Leptin Protein (His Tag)

Sequence: Pro22-Cys171

Characteristics: Recombinant Carassiusauratus Leptin is produced by our Yeast expression system and the target gene encoding Pro22-Cys171 is expressed with a 8His tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Citrate, 8 % Trehalose, 4 % Mannitol, 0.02 % Tween 80 (w/v), pH 5.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Leptin (LEP)

Alternative Name: Leptin

Background: LEP,Leptin,OB,OBS,Obese Protein,Obesity Factor,Leptin is a hormone secreted from white adipocytes and plays important role in the regulation of food intake and energy balance. Leptin functions via signaling pathways involving OB-R in hypothalamus. In mammals, leptin is mainly produced by the adipose tissue and encodes body fat reserves, acting as a short-term satiety signal. In fish, the presence of a leptin-like peptide was first evidenced by immuno-cross-reactivity, and its existence was certainly demonstrated after the finding by synteny of a leptin sequence in the pufferfish.

Molecular Weight:

Calculated MW: 18.3 kDa

Observed MW: 17 kDa

UniProt: B8YI02

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process