Leptin Protein (LEP) (AA 22-167) (hFc Tag)

Catalog No. ABIN7275142

This Recombinant Leptin protein is expressed in HEK-293 Cells.

Quick Overview for Leptin Protein (LEP) (AA 22-167) (hFc Tag) (ABIN7275142)

Target: Leptin (LEP)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-167

Purification tag / Conjugate: This Leptin protein is labelled with hFc Tag.

Purpose: Mouse Leptin Protein

Sequence: Val22-Cys167

Characteristics: Recombinant Mouse Leptin Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Val22-Cys167.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse Leptin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse Leptin is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Leptin (LEP)

Alternative Name: Leptin

Background: Leptin has key roles in the regulation of energy balance, body weight, metabolism, and endocrine function. Leptin levels are undetectable or very low in patients with lipodystrophy, hypothalamic amenorrhea, and congenital leptin deficiency (CLD) due to mutations in the leptin gene.

Molecular Weight: 42.76 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.

UniProt: P41160

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process