Leptin Protein (LEP) (AA 22-167) (Fc Tag)
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- Target See all Leptin (LEP) Proteins
- Leptin (LEP)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-167
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Leptin protein is labelled with Fc Tag.
- Purpose
- Mouse Leptin Protein
- Sequence
- Val22-Cys167
- Characteristics
- Recombinant Mouse Leptin Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Val22-Cys167.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Leptin (LEP) (Active) protein
LEP Origin: Mouse Host: Escherichia coli (E. coli) Recombinant SDS, Func Active
Leptin (LEP) proteinLEP Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by reducing SDS-PAGE.
Leptin (LEP) (AA 22-167) protein (His tag)LEP Origin: Pig Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Leptin (LEP) (AA 22-167) protein (His tag)LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Leptin (LEP) protein (His tag)LEP Origin: Goldfish Host: Yeast Recombinant > 95 % as determined by reducing SDS-PAGE.
Leptin (LEP) (AA 428-635) protein (His tag)LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Leptin (LEP) (AA 22-167) protein (GST tag)LEP Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Leptin (LEP) (AA 2-147) proteinLEP Origin: Mouse Host: Escherichia coli (E. coli) Recombinant SDS
Leptin (LEP) (AA 29-164), (partial) protein (GST tag)LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Leptin (LEP)
- Alternative Name
- Leptin (LEP Products)
- Synonyms
- ob Protein, obese Protein, LEPD Protein, OB Protein, OBS Protein, leptin Protein, Lep Protein, LEP Protein, lep Protein
- Background
- Leptin has key roles in the regulation of energy balance, body weight, metabolism, and endocrine function. Leptin levels are undetectable or very low in patients with lipodystrophy, hypothalamic amenorrhea, and congenital leptin deficiency (CLD) due to mutations in the leptin gene.
- Molecular Weight
- 42.76 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.
- UniProt
- P41160
- Pathways
- JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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