Leptin Protein (LEP) (AA 22-167)

Catalog No. ABIN7320902

This Recombinant Leptin protein is produced in Escherichia coli (E. coli).

Quick Overview for Leptin Protein (LEP) (AA 22-167) (ABIN7320902)

Target: Leptin (LEP)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: ELISA, Bio-Layer Interferometry (BLI)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 22-167

Purpose: Recombinant Mouse Leptin

Sequence: Val22-Cys167

Characteristics: Recombinant Mouse Leptin is produced by our E.coli expression system and the target gene encoding Val22-Cys167 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized Recombinant Mouse LEPR (C-10His)(PKSM041415) at 5μg/ml (100 μl/well) can bind Recombinant Mouse Leptin(PKSM041419): Biotinylated by NHS-biotin prior to testing.The ED₅₀ of Recombinant Mouse Leptin(PKSM041419) is 1.16 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 300 mM NaCl, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Leptin (LEP)

Alternative Name: Leptin

Background: LEP,Leptin,OB,OBS,Obese Protein,Obesity Factor,Leptin is a hormone secreted from white adipocytes and plays important role in the regulation of food intake and energy balance. Leptin functions via signaling pathways involving OB-R in hypothalamus. Animal models have revealed the influence of Leptin in reducing body weight and regulating blood glucose level. When mutations are introduced in obese gene, mice with impaired function of leptin are massively obese and in high risk of diabetes. Leptin deficiency reduces metablic rate. Leptin deficient mice are less active and with lower body temperature than normal animals. Human Leptin shares approximately 84 % sequence identity with the mouse protein. Human Leptin consists of 167 amino acid residue including a 21 amino acid residue signal sequence and 146 amino acid residue mature protein sequence.

Molecular Weight:

Calculated MW: 16.1 kDa

Observed MW: 14 kDa

UniProt: Q544U0

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process