SIRT1 Protein (AA 510-747) (His tag)

Catalog No. ABIN7125104

This Recombinant SIRT1 protein is produced in Escherichia coli (E. coli).

Quick Overview for SIRT1 Protein (AA 510-747) (His tag) (ABIN7125104)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 510-747

Purification tag / Conjugate: This SIRT1 protein is labelled with His tag.

Purpose: Recombinant Human SIRT1

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: SIRT1

Background: EC 3.5.1, S. cerevisiae, homolog) 1, SIR2alpha, SIR2L1, SIR2-like protein 1, SIRT1, Sirtuin 1

Molecular Weight: 27.9 kDa

UniProt: Q96EB6

Pathways: MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling