SIRT1 Protein (AA 510-747) (His tag)

Catalog No. ABIN7125104

Product Details

Target: SIRT1 (Sirtuin 1 (SIRT1))

Protein Type: Recombinant

Protein Characteristics: AA 510-747

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This SIRT1 protein is labelled with His tag.

Application: Western Blotting (WB), ELISA

Purity: Greater than 95 % by SDS-PAGE gel analyses

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/mL in sterile distilled water by gentle pipetting 2-3 times, don't vortex

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % trehalose, pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: -20°C for 12 months as lyophilized, 2-8°C for 1 month under sterile conditions after reconstitution

Expiry Date: 12 months

Target Details

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: SIRT1 (SIRT1 Products)

Background: EC 3.5.1, S. cerevisiae, homolog) 1, SIR2alpha, SIR2L1, SIR2-like protein 1, SIRT1, Sirtuin 1

Molecular Weight: 27.9 kDa

UniProt: Q96EB6

Pathways: MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling