SMAD3 Protein (AA 33-178) (His-IF2DI Tag)

Catalog No. ABIN7125123

This Recombinant SMAD3 protein is expressed in Escherichia coli (E. coli).

Quick Overview for SMAD3 Protein (AA 33-178) (His-IF2DI Tag) (ABIN7125123)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 33-178

Purification tag / Conjugate: This SMAD3 protein is labelled with His-IF2DI Tag.

Purpose: Recombinant Human SMAD3

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: SMAD3

Background: HMAD 3, hSMAD3, HSPC193, HsT17436, JV15 2, MAD homolog 3, Mad3, MADH3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3, SMAD3

Molecular Weight: 37.5 kDa

UniProt: P84022

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy