Laminin beta 1 Protein (full length) (rho-1D4 tag)

Catalog No. ABIN7126007

Product Details

Target: Laminin beta 1 (LAMB1) (Laminin, beta 1 (LAMB1))

Protein Type: Recombinant

Protein Characteristics: full length

Origin: CHO cells

Source: Insect Cells

Purification tag / Conjugate: This Laminin beta 1 protein is labelled with rho-1D4 tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys), Functional Studies (Func)

Sequence: MLIERSSDFG KTWGVYRYFA YDCESSFPGI STGPMKKVDD IICDSRYSDI EPSTEGEVIF RALDPAFKIE DPYSPRIQNL LKITNLRIKF VKLHTLGDNL LDSRMEIREK YYYAVYDMVV RGNCFCYGHA SECAPVDGID EAVEGMVHGH CMCRHNTKGL NCELCMDFYH DLPWRPAEGR NSNACKKCNC NEHSSSCHFD MAVFLATGNV SGGVCDDCQH NTMGRNCEQC KPFYYQHPER DVRDPNLCEP GSENGGICDG YTDFSAGLIA GQCRCKLHVE GERCDVCKEG FYGLSAEDPF GCKSCACNPL GTIPGGNPCD SETGYCYCKR LVTGQRCDQC LPQHWGLSND LDGCRPCDCD LGGALNNSCS EDSGQCSCLP HMIGRQCNEV ESGYYFTTLD HYIYEAEDAN LGPGVIVVER QYIQDRIPSW TGPGFVRVPE GAYLEFFIDN IPYSMEYDIL IRYEPQLPDH WEKAVITIQR PGKIPTSSRC GNTVPDDDNQ VVSLSPGSRY IVLPRPVCFE KGMNYTVRLE LPQYTASGSD VENPYTLIDS LVLMPYCKSL GIFTAGGSGD GEVTNSAWET FQRYRCLENS RSVVKTPMTD VCRNIIFSIS AMIHQTGLAC ECDPQGSLSS VCDPNGGQCQ CRPNVVGRAC NRCAPGTFGF GPNGCKPCDC DLQGSVDAFC DAVTGQCHCF QGIYARQCDR CLPGHWGFPS CQPCQCNGHA EDCDTVTGEC LSCQDYTTGH NCERCLAGYY GDPIIGSGDH CRPCPCPDGP NSGRQFARSC YQDPITLQLA CVCDPGYIDF GGSCQPCQCH HNIDTTDPEA CDKETGRCLK CLYHTEGDHC QLCQYGYYGD ALRQDCRKCV CNYLGTVQEH CNGSDCHCDK ATGQCLCLPN VIGQNCDQCA PNTWQLASGT GCESCNCNAA HSFGPSCNEF TGQCQCMPGF GGRTCSECQE LFWGDPDVEC RACDCDPRGI ETPQCDQSTG QCVCVEGVEG PRCDKCTRGY SGVFPDCTPC HQCFALWDVI IGELTNRTHK FLEKAKALKI SGVIGPYRET VDSVEKKVNE IKDILAQSPA AEPLKNIGNL FEEAEKLTKD VSEKMAQVEM RLSDTASQSN STAGELAALQ AEAKSLEKTV KELAEQLEFI KNSDIQGALD SVTKYFQISL EAEKRVNAST TDPNSTVEQS ALTRDRVEDL MLERESQFKE EQEEQARLLD ELAGKLQSLD LSAAAEMTCG TPPGADCSES ECGGPNCRTD EGEKKCGGPG CGGLVTVAHS AWQKAMDFDR DVLSALAEVE QLSKMVSEAK VRADEAKQNA QDVLLKTNAT KEKVDKSNED LRNLIKQIRD FLTEDSADLD SIEAVANEVL KMEMPSTPQQ LQNLTEDIRE RVETLSQVEV ILQQSAADIA RAELLLEEAK RASKSATDVK VTADMVKEAL EEAEKAQIAA EAAIKQADDD IQGTQNLLTS IESETAASEE SLSNASQRIS KLERNVEELK RKAAQNSGEA EYIEKVVYSV KQSADDVKKA LDGELDEKYK KVESLIAQKT EESADARRKA ELLQNEAKTL LAQANSKLQL LEALERKYED NQKYLEDKAQ ELVRLEGEVR SLLKDISEKV AVYSTCL
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• CHO Laminin subunit beta-1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.

Grade: Crystallography grade

Biological Activity Comment: Protein has not been tested for activity yet.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Laminin beta 1 (LAMB1) (Laminin, beta 1 (LAMB1))

Alternative Name: Laminin subunit beta-1 (LAMB1 Products)

Synonyms: CLM Protein, LIS5 Protein, fc20b05 Protein, gup Protein, lamb1 Protein, sb:cb95 Protein, wu:fc20b05 Protein, Lamb1-1 Protein, LAMB1 Protein, C77966 Protein, C80098 Protein, C81607 Protein, D130003D08Rik Protein, Lamb-1 Protein, laminin subunit beta 1 Protein, laminin, beta 1a Protein, laminin B1 Protein, LAMB1 Protein, lamb1a Protein, Lamb1 Protein, lamb1 Protein

UniProt: G3I278