TKT Protein (full length) (rho-1D4 tag)

Catalog No. ABIN7126021

Product Details

Target: TKT (Transketolase (TKT))

Protein Type: Recombinant

Protein Characteristics: full length

Origin: CHO cells

Source: Insect Cells

Purification tag / Conjugate: This TKT protein is labelled with rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys), Functional Studies (Func)

Sequence: MEGYHKPDQQ KLQALKDTAN RLRISSIQAT TAAGSGHPTS CCSAAEIMAV LFFHTMRYKA LDPRNPHNDR FVLSKGHAAP ILYAVWAEAG FLPETELLNL RKISSDLDGH PVPKQAFTDV ATGSLGQGLG AACGMAYTGK YFDKASYRVY CMLGDGEVSE GSVWEAMAFA GIYKLDNLVA IFDVNRLGQS DPAPLQHQLD VYQKRCEAFG WHAIIVDGHS VEELCKAFGQ AKHQPTAIIA KTFKGRGITG IEDKESWHGK PLPKNMADQI IQEIYSQIQS KKKILATPPQ EDAPSVDIAN IRMPTPPSYK VGDKIATRKA YGQALAKLGH ASDRIIALDG DTKNSTFSDI FKKEHPDRFI ECYIAEQNMV SIAVGCATRN RTVPFCSTFA AFFTRAFDQI RMAAISESNI NLCGSHCGVS IGEDGPSQMA LEDLAMFRSI PMSTVFYPSD GVATEKAVEL AANTKGICFI RTSRPENAII YSNNEDFQVG QAKVVLKSKD DQVTVIGAGV TLHEALAAAE LLKKEKISIR VLDPFTIKPL DRKLILECAR ATKGRILTVE DHYYEGGIGE AVSAAVVGEP GVTVTRLAVS QVPRSGKPAE LLKMFGIDKD AIVQAVKGLV TKG
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• CHO Transketolase Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.

Grade: Crystallography grade

Biological Activity Comment: Protein has not been tested for activity yet.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: TKT (Transketolase (TKT))

Alternative Name: Transketolase (TKT Products)

Synonyms: TK Protein, TKT1 Protein, p68 Protein, Dmel\\CG8036 Protein, anon-WO0118547.344 Protein, cb860 Protein, fb38f03 Protein, fj52f12 Protein, id:ibd3270 Protein, wu:cegs2794 Protein, wu:fb38f03 Protein, wu:fj52f12 Protein, tkt1 Protein, PSPTO0385 Protein, Tb08.11J15.550 Protein, xcc-b100_0966 Protein, transketolase Protein, CG8036 gene product from transcript CG8036-RB Protein, TransKeTolase homolog Protein, transketolase b Protein, transketolase L homeolog Protein, transketolase Tkt Protein, tkt Protein, TKT Protein, Tkt Protein, CG8036 Protein, tkt-1 Protein, tktb Protein, tkt.L Protein, tkt Protein, JK_RS05135 Protein, Tb927.8.6170 Protein, APH_RS01480 Protein

UniProt: G3GUU5