Pyruvate Kinase Protein (PK) (full length) (rho-1D4 tag)
Crystallography grade
PK
Origin: CHO cells
Host: Insect Cells
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Crys, ELISA, Func, SDS, WB
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- Target See all Pyruvate Kinase (PK) products
- Pyruvate Kinase (PK)
- Protein Type
- Recombinant
- Protein Characteristics
- full length
- Origin
- CHO cells
- Source
- Insect Cells
- Purification tag / Conjugate
- This Pyruvate Kinase protein is labelled with rho-1D4 tag.
- Application
- Crystallization (Crys), ELISA, Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MLKEMIKSGM NVARLNFSHG THEYHAETIK NVRTATESFA SDPILYRPVA VALDTKGPEI RTGLIKGSGT AEVELKKGAT LKITLDNAYM EKCDENILWL DYKNICKVVE VGSKIYVDDG LISLQVKEKG ADYLVTEVEN GGSLGSKKGV NLPGAAVDLP AVSEKDIQDL KFGVEQGVDM VFASFIRKAE DVHEVRKVLG EKGQNIKIIS KIENHEGVRR FDEILEASDG IMVARGDLGI EIPAEKVFLA QKMMIGRCNR AGKPVICATQ MLESMIKKPR PTRAESSDVA NAVLDGADCI MLSGETAKGD YPLEAVRMQH LIAREAEAAI YHLQLFEELR RLAPITNDPT EAAAVGAVEA SFKCCSGAII VLTKSGRSAH QVARYRPRAP IIAVTRNPQT ARQAHLYRGI FPVLCKDAVQ DAWAEDVDLR VNLAMNVGKA RGFFKTGDVV IVLTGWRPGS GYTNTMRVVP VP
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- CHO Pyruvate kinase Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Three step purification of proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.
- Grade
- Crystallography grade
- Biological Activity Comment
- Protein has not been tested for activity yet.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- Pyruvate Kinase (PK)
- Alternative Name
- Pyruvate kinase (PK Products)
- Synonyms
- CG7070 Protein, Dmel\\CG7070 Protein, PYK Protein, Pyk Protein, PYK3b Protein, PYK3c Protein, Pyruvate kinase Protein, PK Protein, pyruvate kinase Protein, pyruvate kinase (PK) Protein, Pyruvate kinase (PK) Protein, pyruvate kinase PKM Protein, testis expressed 45 Protein, PyK Protein, pykA Protein, PYK3a|PYK3b|PYK3c Protein, pyk Protein, pyk1 Protein, pk Protein, LOC100534543 Protein, TEX45 Protein
- UniProt
- G3H3Q1
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