HSP90AB1 Protein (full length) (rho-1D4 tag)

Catalog No. ABIN7126044

Product Details

Target: HSP90AB1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1))

Protein Type: Recombinant

Protein Characteristics: full length

Origin: CHO cells

Source: Insect Cells

Purification tag / Conjugate: This HSP90AB1 protein is labelled with rho-1D4 tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys), Functional Studies (Func)

Sequence: MYYWVFQHFS VEGQLEFRAL LFIPRRAPFD LFENKKKKNN IKLYVRRVFI MDSCDELIPE YLNFIRGVVD SEDLPLNISR EMLQQSKILK VIRKNIVKKC LELFSELAED KENYKKFYEA FSKNLKLGIH EDSTNRRRLS ELLRYHTSQS GDEMTSLSEY VSRMKETQKS IYYITGESKE QVANSAFVER VPKRGFEAVY MTEPIDEYCV QQLKEFDGKS LVSVTKEGLE LPEDEEEKKK MEESKAKFEN LCKLMKEILD KKVEKVTISN RLVSSPCCIV TSTYGWTANM ERIMKAQALR DNSTMGYMMA KKHLEINPDH PIVETLRQKA EADKNDKAVK DLVVLLFETA LLSSGFSLED PQTHSNRIYR MIKLGLGIDE DEVTAEEPSA AVPDEIPPLE GDEDASRMEE VD
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• CHO Heat shock protein HSP 90-beta Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.

Grade: Crystallography grade

Biological Activity Comment: Protein has not been tested for activity yet.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: HSP90AB1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1))

Alternative Name: Heat shock protein HSP 90-beta (HSP90AB1 Products)

Synonyms: D6S182 Protein, HSP84 Protein, HSP90B Protein, HSPC2 Protein, HSPCB Protein, GRP94 Protein, TRA1 Protein, hsp90b Protein, 90kDa Protein, AL022974 Protein, C81438 Protein, Hsp84 Protein, Hsp84-1 Protein, Hsp90 Protein, Hspcb Protein, Hsp70 Protein, Hsp70-1 Protein, Hsp70.1 Protein, hsp68 Protein, HSP90-BETA Protein, hsp90beta Protein, wu:fa29f01 Protein, wu:fa91e11 Protein, wu:fd59e11 Protein, wu:gcd22h07 Protein, HSP90 Protein, heat shock protein 90 alpha family class B member 1 Protein, heat shock protein 90 beta family member 1 Protein, Heat Shock Protein 90, endoplasmic reticulum Protein, heat shock protein 90B Protein, heat shock protein 90 alpha (cytosolic), class B member 1 Protein, heat shock protein 1B Protein, heat shock protein 90kDa alpha family class B member 1 S homeolog Protein, heat shock protein 90, alpha (cytosolic), class B member 1 Protein, HSP90AB1 Protein, HSP90B1 Protein, HSP90B Protein, hsp90ab1 Protein, Hsp90ab1 Protein, Hspa1b Protein, hsp90ab1.S Protein

UniProt: G3HC84

Pathways: Regulation of Cell Size