Malate Dehydrogenase (MDH) (full length) protein (rho-1D4 tag)
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- Target See all Malate Dehydrogenase (MDH) Proteins
- Malate Dehydrogenase (MDH)
- Protein Type
- Recombinant
- Protein Characteristics
- full length
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Origin
- CHO cells
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Source
- Insect Cells
- Purification tag / Conjugate
- rho-1D4 tag
- Application
- ELISA, Crystallization (Crys), Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC ALPLLKDVIA TDKEEVAFKD LDVAVLVGSM PRREGMERKD LLKANVRIFK AQGAALEKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE NFSCLTRLDH NRAKSQIALK LGVTAEDVKN VIIWGNHSST QYPDVNHAKV KLQGKEVGVY EALKDDSWLK GEFISTVQQR GAAVIKARKL SSAMSAAKAI SDHIRDIWFG TPEGEFVSMG VISDGNSYGI PDDLLYSFPV VIKDKSWKFV EGLPINDFSR EKMDLTAKEL AEEKETAFEF LSSA
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- CHO Malate dehydrogenase Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Three step purification of proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.
- Grade
- Crystallography grade
- Biological Activity Comment
- Protein has not been tested for activity yet.
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Malate Dehydrogenase (MDH) (AA 2-312) protein (His tag)
MDH Origin: Bacillus subtilis Host: Yeast Recombinant > 90 % ELISA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- Malate Dehydrogenase (MDH)
- Alternative Name
- Malate dehydrogenase (MDH Products)
- Synonyms
- CG5889 Protein, Dmel\\CG5889 Protein, MDH Protein, Mdh Protein, anon-EST:Posey79 Protein, MALATE DEHYDROGENASE Protein, malate dehydrogenase Protein, An15g00070 Protein, AO090701000013 Protein, DDBDRAFT_0188779 Protein, DDBDRAFT_0230186 Protein, DDB_0188779 Protein, DDB_0230186 Protein, Malic enzyme b Protein, oxidoreductase, putative Protein, malate dehydrogenase Protein, NAD dependent malate dehydrogenase Mdh Protein, Men-b Protein, malate dehydrogenase Protein, MDH Protein, mdh Protein, APH_RS02635 Protein, ANI_1_12134 Protein, AOR_1_26114 Protein, PMI_RS16925 Protein, mdhA Protein, mdh1 Protein
- UniProt
- G3HDQ2
- Pathways
- Regulation of Lipid Metabolism by PPARalpha
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