RPLP0 Protein (full length) (rho-1D4 tag)

Catalog No. ABIN7126061

Product Details

Target: RPLP0 (Ribosomal Protein, Large, P0 (RPLP0))

Protein Type: Recombinant

Protein Characteristics: full length

Origin: CHO cells

Source: Insect Cells

Purification tag / Conjugate: This RPLP0 protein is labelled with rho-1D4 tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys), Functional Studies (Func)

Sequence: MPREDRATWK SNYFLKIIQL LDDYPKCFIV GADNADSKQM QQIRMSLQGK AVVLMGKNTM MRKAIRGHLE NNPALEKLLP HIRGNVGFVF TKEDLTEIRD MLLANKVPAA ARAGAIAPCK VTVPAQNTGL GPEKTSFFQA LGITTKTSRG TIEILSDVQL IKTGDKVGAS EATLLNMLNI SSFSFGLIIQ QVFDNSSIYN PEVLDVTEQT LHSRFLEGVR NVVSGCLQIG YPTVASVPHS IINGYKRGLA LSVETEYTFP LAEKVKAFLA DPSTFAAAAL
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• CHO 60S acidic ribosomal protein P0 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.

Grade: Crystallography grade

Biological Activity Comment: Protein has not been tested for activity yet.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RPLP0 (Ribosomal Protein, Large, P0 (RPLP0))

Alternative Name: 60S acidic ribosomal protein P0 (RPLP0 Products)

Synonyms: AP3 Protein, Ap3 Protein, Ape Protein, CG7490 Protein, Dmel\\CG7490 Protein, LP0 Protein, P0 Protein, P35 Protein, PO Protein, RLA0 Protein, RPL P0 Protein, RpP0 Protein, RpPO Protein, Rplp0 Protein, l(3)0154 Protein, l(3)01544 Protein, p0 Protein, RPLP0 Protein, arp Protein, fa56b12 Protein, fb04a12 Protein, wu:fa56b12 Protein, wu:fb15a08 Protein, wu:fk48c12 Protein, arbp Protein, l10e Protein, lp0 Protein, prlp0 Protein, rpp0 Protein, L10E Protein, PRLP0 Protein, RPP0 Protein, 36B4 Protein, Arbp Protein, ARBP Protein, Ribosomal protein LP0 Protein, ribosomal protein lateral stalk subunit P0 Protein, ribosomal protein, large, P0 Protein, ribosomal protein, large, P0 S homeolog Protein, RpLP0 Protein, RPLP0 Protein, rplp0 Protein, rplp0.S Protein, Rplp0 Protein

Background: Ribosomal protein P0 is the functional equivalent of E.coli protein L10. {ECO:0000256|ARBA:ARBA00002200, ECO:0000256|PIRNR:PIRNR039087}.

UniProt: G3HKG9