GDF15 Protein (His-Avi Tag,Biotin)

Catalog No. ABIN7199635

Recombinant GDF15 protein expressed in HEK-293 Cells.

Quick Overview for GDF15 Protein (His-Avi Tag,Biotin) (ABIN7199635)

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: >90 % as determined by SDS-PAGE.

Grade: MALS verified

Product Details

Purification tag / Conjugate: This GDF15 protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human GDF-15 / MIC-1 Protein, Avitag™, Fc Tag (MALS verified)

Sequence: Ala 197 - Ile 308

Characteristics: Biotinylated Human GDF-15, Avitag, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ala 197 - Ile 308 (Accession # Q99988-1).

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag. The protein has a calculated MW of 40.4 kDa. As a result of glycosylation, the protein migrates as 50-55 kDa under reducing (R) condition, and 80-90 kDa under no

Comment:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Target Details

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Alternative Name: GDF-15 / MIC-1

Background: Synonyms: GDF15,GDF-15,MIC-1,MIC1,NAG-1,PDF,PLAB,PTGFB,NRG-1,
Description: Growth Differentiation Factor 15 (GDF-15), also called Macrophage Inhibitory Cytokine 1 (MIC-1). Expression of MIC-1 mRNA in monocytoid cells is up-regulated by a variety of stimuli associated with activation, including interleukin 1β, tumor necrosis factor α (TNF-α), interleukin 2, and macrophage colony-stimulating factor but not interferon γ, or lipopolysaccharide (LPS). It is highly expressed in cardiomyocytes, adipocytes, macrophages, endothelial cells, and vascular smooth muscle cells in normal and pathological condition. GDF-15 increases during tissue injury and inflammatory states and is associated with cardiometabolic risk. Increased GDF-15 levels are associated with cardiovascular diseases such as hypertrophy, heart failure, atherosclerosis, endothelial dysfunction, obesity, insulin resistance, diabetes, and chronic kidney diseases in diabetes. Increased GDF-15 level is linked with the progression and prognosis of the disease condition.

Molecular Weight: 40.4 kDa

NCBI Accession: NP_004855

Pathways: SARS-CoV-2 Protein Interactome