AFT1 Protein (His tag)
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- Target See all AFT1 Proteins
- AFT1 (Activating Transcription Factor 1 (AFT1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This AFT1 protein is labelled with His tag.
- Purpose
- Recombinant Human ATF1 Protein (His Tag)
- Sequence
- Met 1-Val271
- Characteristics
- Recombinant Human Activating Transcription Factor 1 is produced by our E.coli expression system and the target gene encoding Met1-Val271 is expressed with a 6His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product AFT1 Protein
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Activating Transcription Factor 1 (AFT1) (AA 1-271) protein (GST tag)
AFT1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Activating Transcription Factor 1 (AFT1) (AA 121-220) protein (GST tag)AFT1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Activating Transcription Factor 1 (AFT1) (AA 1-269) protein (Strep Tag)Crystallography grade AFT1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Activating Transcription Factor 1 (AFT1) proteinAFT1 Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, ELISA, IP
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- AFT1 (Activating Transcription Factor 1 (AFT1))
- Alternative Name
- ATF1 (AFT1 Products)
- Synonyms
- ews-atf1 Protein, fus/atf-1 Protein, treb36 Protein, ATF1 Protein, EWS-ATF1 Protein, FUS/ATF-1 Protein, TREB36 Protein, Atf-1 Protein, wu:fc92c07 Protein, zgc:64099 Protein, activating transcription factor 1 Protein, activating transcription factor 1 L homeolog Protein, atf1 Protein, ATF1 Protein, MGC85066 Protein, atf1.L Protein, Atf1 Protein
- Background
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Background: Cyclic AMP-dependent transcription factor ATF-1(ATF1) which contains 1 bZIP (basic-leucine zipper) domain and 1 KID (kinase-inducible) domain, belongs to the bZIP family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. ATF1 binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It also binds to the Tax-responsive element (TRE) of HTLV-I. ATF1 mediates PKA-induced stimulation of CRE-reporter genes, represses the expression of FTH1 and other antioxidant detoxification genes, triggers cell proliferation and transformation. ATF1 is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and CDK3. Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation.
Synonym: Cyclic AMP-dependent transcription factor ATF-1,cAMP-dependent transcription factor ATF-1,Activating transcription factor 1,
- Molecular Weight
- 30.3 kDa
- UniProt
- P18846
- Pathways
- Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades
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