ALT Protein (His tag)

Catalog No. ABIN7194188

Recombinant ALT protein expressed in Baculovirus infected Insect Cells.

Quick Overview for ALT Protein (His tag) (ABIN7194188)

Target: ALT (Alanine Aminotransferase (ALT))

Protein Type: Recombinant

Origin: Rat

Source: Baculovirus infected Insect Cells

Purity: > 95 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This ALT protein is labelled with His tag.

Purpose: Recombinant Rat GPT1/GPT Protein (His Tag)

Sequence: Met 1-Ser 496

Characteristics: A DNA sequence encoding the rat Gpt1 (NP_112301.1) (Met 1-Ser 496) with a C-terminal polyhistidine tag was expressed.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 10 % glycerol, 0.5 mM TCEP

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: ALT (Alanine Aminotransferase (ALT))

Alternative Name: GPT1/GPT

Background:

Background: Alanine aminotransferase (ALT), also known as glutamate pyruvate transaminase (GPT), is a pyridoxal enzyme which belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily. Gpt / Gpt1 / ALT catalyses the reversible interconversion of L-alanine and 2-oxoglutalate to pyruvate and L-glutamate, and plays a key role in the intermediary metabolism of glucose and amino acids. Gpt / Gpt1 / ALT is expressed in Liver, kidney, heart, and skeletal muscles and it expresses at moderate levels in the adipose tissue. As a key enzyme for gluconeogenesis, Gpt is a widely-used serum marker for liver injury. Two ALT isoenzymes have been identified, ALT1 and ALT2 (GPT1 and GPT2), which are encoded by separate genes and share significant sequence homology, but differ in their expression patterns. GPT1/GPT is widely distributed and mainly expressed in intestine, liver, fat tissues, colon, muscle, and heart, in the order of high to low expression level. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis.

Synonym: Gpt1,Gpt,ALT1

Molecular Weight: 55 kDa

NCBI Accession: NP_112301