CLEC4F Protein
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- Target See all CLEC4F Proteins
- CLEC4F (C-Type Lectin Domain Family 4, Member F (CLEC4F))
- Protein Type
- Recombinant
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Origin
- Rat
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Source
- HEK-293 Cells
- Purpose
- Recombinant Rat CLEC4F/CLECSF13 Protein
- Sequence
- Arg70-Ser550
- Characteristics
- A DNA sequence encoding the rat CLEC4F(NP_446205.1) (Arg70-Ser550) was expressed with two additional amino acids (Gly & Pro) at the N-terminus.
- Purity
- > 95 % as determined by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method
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C-Type Lectin Domain Family 4, Member F (CLEC4F) (AA 1-589) protein (Strep Tag)
Crystallography grade CLEC4F Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
C-Type Lectin Domain Family 4, Member F (CLEC4F) (AA 1-548) protein (Strep Tag)Crystallography grade CLEC4F Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
C-Type Lectin Domain Family 4, Member F (CLEC4F) (AA 61-589) protein (His tag)Crystallography grade CLEC4F Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
C-Type Lectin Domain Family 4, Member F (CLEC4F) (AA 70-548) protein (His tag)Crystallography grade CLEC4F Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- CLEC4F (C-Type Lectin Domain Family 4, Member F (CLEC4F))
- Alternative Name
- CLEC4F/CLECSF13 (CLEC4F Products)
- Synonyms
- CLECSF13 Protein, KCLR Protein, Clecsf13 Protein, D18063 Protein, KUCR_MOUSE Protein, Kclr Protein, C-type lectin domain family 4 member F Protein, C-type lectin domain family 4, member f Protein, C-type lectin domain family 4, member F Protein, CLEC4F Protein, Clec4f Protein
- Background
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Background: CLEC4F, a member of C-type lectins, was firstly purified from rat liver extract with high binding affinity to fucose, galactose and N-acetylgalactosamine, and un-sialylated glucosphingolipids with GalNAc or Gal terminus. However, the biological functions of CLEC4F have not been elucidated. Histochemical staining showed that mouse CLEC4F(mCLEC4F) is only expressed on F4/80+ cells localized in liver, and is undetectable in bone marrow, spleen, lymph nodes, or other tissues in adult mice. However, mCLEC4F is detected in the liver of embryonic day 11.5 (E11.5), which is 1.5 day earlier than the formation of liver (E10) and is 3.5 day earlier than the formation of bone marrow (E15-16). Moreover, recombinant mCLEC4F.Fc binds to alpha-galactoceramide in a Ca++-dependent manner, and both galactose and ceramide can partially inhibit CLEC4F.Fc binding to alpha-galactoceramide. Interestingly, mCLEC4F-deficient (mCEC4F k/o) mice produced far less cytokines than wild type littermates after intravenous injection ofalpha-galactoceramide. This suggests that mCLEC4F is not only a specific marker for Kupffer cells, but is also critical for the presentation of glycolipid antigen to NKT cells.
Synonym: CLEC4F,Clecsf13,Kclr
- Molecular Weight
- 53.7 kDa
- NCBI Accession
- NP_446205
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