Cadherin 4 Protein (CDH4) (His tag)

Catalog No. ABIN7317950

Product Details

Target: Cadherin 4 (CDH4)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Cadherin 4 protein is labelled with His tag.

Purpose: Recombinant Human R-Cadherin/CDH4 Protein (His Tag)(Active)

Sequence: Met 1-Ala 734

Characteristics: A DNA sequence encoding the extracellular domain of human CAD4 (NP_001785.2) (Met 1-Ala 734) was expressed with a fused polyhistidine tag at the C-terminus.

Purity: > 85 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. Immobilized CAD4 (0.8 μg/ml, 100 μl/well) will mediate >20% C6 cell adhesion.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Cadherin 4 (CDH4)

Alternative Name: R-Cadherin/CDH4 (CDH4 Products)

Synonyms: cad4 Protein, rcad Protein, R-cadherin Protein, cadherin-4 Protein, AW120700 Protein, R-Cadh Protein, Rcad Protein, Cdh4l Protein, zgc:136316 Protein, CAD4 Protein, RCAD Protein, cadherin 4 Protein, cadherin 4, type 1, R-cadherin (retinal) Protein, Cadherin-4 Protein, cdh4 Protein, Cdh4 Protein, CDH4 Protein, cdh-4 Protein

Background:

Background: The cadherin superfamily is a large family that engage in both homo- and heterotypic, calcium-dependent, cell-cell adhesion events, and can be divided into at least four subfamilies based on the extracellular (EC) regions and cytoplasmic domains, that is: classical cadherins, desmosomal cadherins, protocadherins, and cadherin-like molecules. Human cadherin 4, type 1, R-cadherin (retinal), also known as CDH4, CAD4 and RCAD, is a classical cadherin from the cadherin superfamily. It is a calcium-dependent adhesion molecule and a type I  transmembrane glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. CDH4 is thought to play an important role during brain segmentation and neuronal outgrowth, and also exerts critical actions in kidney and muscle development. CDH4 is expressed in vascular smooth muscle, pancreatic β-cells, thyroid follicular cells, sensory neurons of the dorsal root ganglia, and, possibly, astrocytes and endothelium of the retina. As a classic cadherin, CDH4 forms both homodimers and heterodimers with N-cadherin. The extracellular region of human CDH4 is 96% aa identical to that of mouse CDH4.

Synonym: CAD4;R-CAD;RCAD

Molecular Weight: 80 kDa

NCBI Accession: NP_001785

Pathways: Cell-Cell Junction Organization, Regulation of Cell Size