CANT1 Protein (His tag)

Catalog No. ABIN7194523

Product Details

Target: CANT1 (Calcium Activated Nucleotidase 1 (CANT1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CANT1 protein is labelled with His tag.

Purpose: Recombinant Human CANT1 Protein (His Tag)

Sequence: Gly 80-Ile 401

Characteristics: A DNA sequence encoding the human CANT1 (Q8WVQ1-1) extracellular domain (Gly 80-Ile 401) was fused with polyhistidine tag at the N-terminus.

Purity: > 88 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CANT1 (Calcium Activated Nucleotidase 1 (CANT1))

Alternative Name: CANT1 (CANT1 Products)

Synonyms: DBQD Protein, SCAN-1 Protein, SCAN1 Protein, SHAPY Protein, 5830420C20Rik Protein, Apy1h Protein, D11Bwg0554e Protein, Entpd8 Protein, Shapy Protein, srapy Protein, apyrase Protein, xapy Protein, cant1 Protein, entpd8 Protein, zgc:85724 Protein, CANT1 Protein, zgc:101021 Protein, calcium activated nucleotidase 1 Protein, calcium activated nucleotidase 1 L homeolog Protein, calcium activated nucleotidase 1a Protein, calcium activated nucleotidase 1b Protein, CANT1 Protein, Cant1 Protein, cant1.L Protein, cant1 Protein, cant1a Protein, LOAG_01305 Protein, cant1b Protein

Background:

Background: CANT1(calcium activated nucleotidase 1) belongs to the apyrase family. Apyrase is a calcium-activated plasma membrane-bound enzyme (magnesium can also activate it) (EC 3.6.1.5) that catalyses the hydrolysis of ATP to yield AMP and inorganic phosphate. Two isoenzymes are found in commercial preparations from S. tuberosum. One with a higher ratio of substrate selectivity for ATP: ADP and another with no selectivity. It can also act on ADP and other nucleoside triphosphates and diphosphates with the general reaction being NTP -> NDP + Pi -> NMP + 2Pi. The salivary apyrases of blood-feeding arthropods are nucleotide hydrolysing enzymes are implicated in the inhibition of host platelet aggregation through the hydrolysis of extracellular adenosine diphosphate. CANT1 functions as a calcium-dependent nucleotidase with a preference for UDP. Defects in CANT1 are the cause of desbuquois dysplasia.

Synonym: DBQD;SCAN-1;SCAN1;SHAPY

Molecular Weight: 38 kDa