CAMK1D Protein (GST tag)

Catalog No. ABIN7317091

Product Details

Target: CAMK1D (Calcium/calmodulin-Dependent Protein Kinase ID (CAMK1D))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This CAMK1D protein is labelled with GST tag.

Purpose: Recombinant Human CAMK1D Protein (GST Tag)(Active)

Sequence: Met 1-Lys 385

Characteristics: A DNA sequence encoding the human CAMK1D (NP_705718.1) (Met 1-Lys 385) was fused with the GST tag at the N-terminus.

Purity: > 80 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 70 nmol/min/mg using Autocamtide-2 synthetic peptide (KKALRRQETVDAL-amide) as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 50 mM Tris, 100 mM NaCl, 0.5 mM Reduced Glutathione, pH 8.0

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: CAMK1D (Calcium/calmodulin-Dependent Protein Kinase ID (CAMK1D))

Alternative Name: CAMK1D (CAMK1D Products)

Synonyms: CKLiK Protein, CaM-K1 Protein, CaMKID Protein, A630059D12Rik Protein, CaMKIdelta Protein, E030025C11Rik Protein, CAMK1D Protein, camk1d Protein, zgc:158713 Protein, RGD1560691 Protein, zgc:172284 Protein, cam-ki Protein, camk1d.L Protein, calcium/calmodulin dependent protein kinase ID Protein, calcium/calmodulin-dependent protein kinase ID Protein, calcium/calmodulin-dependent protein kinase 1Db Protein, calcium/calmodulin-dependent protein kinase 1Da Protein, calcium/calmodulin dependent protein kinase ID S homeolog Protein, CAMK1D Protein, Camk1d Protein, camk1db Protein, LOAG_08089 Protein, camk1da Protein, camk1d.S Protein

Background:

Background: Calcium/calmodulin-dependent protein kinase or CaM kinases are serine/threonine-specific protein kinases that are primarily regulated by the Calcium/calmodulin complex. These kinases show a memory effect on activation. CaM kinases activity can outlast the intracellular calcium transient that is needed to activate it. In neurons, this property is important for the induction of synaptic plasticity. Pharmacological inhibition of CaM kinases II blocks the induction of long-term potentiation. Upon activation, CaM kinases II phosphorylates postsynaptic glutamate receptors and changes the electrical properties of the synapse. Calcium/calmodulin-dependent protein kinase type 1D, also known as CaM kinase I delta, CaM kinase ID, CaMKI-like protein kinase, CKLiK and CAMK1D, is a member of the protein kinase superfamily and CaMK subfamily. It contains one protein kinase domain. CAMK1D is broadly expressed. It is highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes. Engineered overexpression of CAMK1D in non-tumorigenic breast epithelial cells led to increased cell proliferation, and molecular and phenotypic alterations indicative of epithelial-mesenchymal transition (EMT), including loss of cell-cell adhesions and increased cell migration and invasion. CAMK1D is a potential therapeutic target with particular relevance to clinically unfavorable basal-like tumors.

Synonym: Calcium/calmodulin-dependent protein kinase type 1D; CaM kinase I delta; CaMKI-like protein kinase; CAMK1D; CaMK1 delta;CaM-K1;CKLiK

Molecular Weight: 69 kDa

NCBI Accession: NP_705718