CAMK1G Protein (GST tag,His tag)

Catalog No. ABIN7317051

Product Details

Target: CAMK1G (Calcium/calmodulin-Dependent Protein Kinase IG (CAMK1G))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This CAMK1G protein is labelled with GST tag,His tag.

Purpose: Recombinant Human CAMK1G/CaMKI gamma Protein (His & GST Tag)

Sequence: Met 1-Met 476

Characteristics: A DNA sequence encoding the human CAMK1G isoform 1 (Q96NX5-1) (Met 1-Met 476) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Purity: > 85 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 20 % glycerol, 0.3 mM DTT

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: CAMK1G (Calcium/calmodulin-Dependent Protein Kinase IG (CAMK1G))

Alternative Name: CAMK1G/CaMKI gamma (CAMK1G Products)

Synonyms: camk1g Protein, wu:fk53c02 Protein, zgc:73127 Protein, CAMK1G Protein, Ci-CaM-K Protein, wu:fk61c03 Protein, zgc:73155 Protein, CLICK-III Protein, CaMKIgamma Protein, CLICK3 Protein, CLICKIII Protein, VWS1 Protein, dJ272L16.1 Protein, calcium/calmodulin-dependent protein kinase IGa Protein, calcium/calmodulin-dependent protein kinase IG Protein, calmodulin-dependent protein kinase homologue Protein, calcium/calmodulin dependent protein kinase IG Protein, calcium/calmodulin-dependent protein kinase IGb Protein, calcium/calmodulin-dependent protein kinase I gamma Protein, camk1ga Protein, CAMK1G Protein, cam-k Protein, camk1gb Protein, Camk1g Protein

Background:

Background: Calmodulin-Dependent Protein Kinase (CaM Kinase) is a kind of protein phosphorylate multiple downstream targets. Concentration of cytosolic calcium functions as a second messenger that mediates a wide range of cellular responses. Calcium binds to calcium binding proteins (calmodulin/CaM) and stimulates the activity of a variety of enzymes, including CaM kinases referred to as CaM-kinases (CaMKs), such as CaMKI, CaMKII, CaMKIV and CaMKK. Calmodulin-dependent protein kinase CL3/CaMKIγ is a memberane-anchored CaMK belonging to the CaM kinase family. Its C-terminal region is uniquely modified by two sequential lipidification steps: prenylation followed by a kinase-activity-regulated palmitoylation. These modifications are essential for CaMKIγ membrane anchoring and targeting into detergent-resistant lipid microdomains in the dendrites. It has been found that CaMKIγ critically contributed to BDNF-stimulated dendritic growth. Raft insertion of CaMKIγ specifically promoted dendritogenesis of cortical neurons by acting upstream of RacGEF STEF and Rac, both present in lipid rafts. Thus, CaMKIγ may represent a key element in the Ca2+-dependent and lipid-raft-delineated switch that turns on extrinsic activity-regulated dendrite formation in developing cortical neurons. 

Synonym: CLICK3;CLICKIII;dJ272L16.1;RP1-272L16.2;VWS1

Molecular Weight: 81 kDa