CEACAM6 Protein (His tag)

Catalog No. ABIN7317753

Product Details

Target: CEACAM6 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (CEACAM6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CEACAM6 protein is labelled with His tag.

Purpose: Recombinant Human CEACAM6/CD66c Protein (His Tag)(Active)

Sequence: Met 1-Gly 320

Characteristics: A DNA sequence encoding the human CEACAM6 (NP_002474.3) (Met 1-Gly 320) with a C-terminal polyhistidine tag was expressed.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized human CEACAM6-his at 10 μg/mL (100 μl/well) can bind biotinylated human CEACAM8-his, The EC50 of biotinylated human CEACAM8-his is 0.17 μg/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CEACAM6 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (CEACAM6))

Alternative Name: CEACAM6/CD66c (CEACAM6 Products)

Synonyms: CD66c Protein, CEAL Protein, NCA Protein, carcinoembryonic antigen-related cell adhesion molecule 6 Protein, carcinoembryonic antigen related cell adhesion molecule 6 Protein, Ceacam6 Protein, CEACAM6 Protein

Background:

Background: Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6), also known as nonspecific crossreacting antigen (NCA) and CD66c, is one of seven human CEACAM family members within the immunoglobulin superfamily. It s a glycosylphosphatidylinositol-linked immunoglobulin superfamily member that is overexpressed in a variety of human cancers, including colon, breast and lung and is associated with tumourigenesis, tumour cell adhesion, invasion and metastasis. CEACAM6 is a unique mediator of migration and invasion of drug resistant oestrogen-deprived breast cancer cells, and this protein could be an important biomarker of metastasis. CEACAM6 is expressed by granulocytes and their progenitors. It is also expressed by epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas, as well as hyperplastic polyps. Resistance to adhesion-related apoptosis in tumor cells is conferred in the condition of CEACAM6 overexpression.

Synonym: CD66c,CEA,CEAL,NCA

Molecular Weight: 32.6 kDa

NCBI Accession: NP_002474