CSNK1G1 Protein (GST tag,His tag)
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- Target See all CSNK1G1 Proteins
- CSNK1G1 (Casein Kinase 1, gamma 1 (CSNK1G1))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This CSNK1G1 protein is labelled with GST tag,His tag.
- Purpose
- Recombinant Human CSNK1G1/CKI-gamma 1 Protein (His & GST Tag)(Active)
- Sequence
- Met 1-Lys 422
- Characteristics
- A DNA sequence encoding full length of human CSNK1G1 (NP_071331.2) (Met 1-Lys 422) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- Purity
- > 94 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- The specific activity was determined to be 4 nmol/min/mg using casein as substrate.
- Top Product
- Discover our top product CSNK1G1 Protein
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Casein Kinase 1, gamma 1 (CSNK1G1) (AA 1-422) (Active) protein (GST tag)
CSNK1G1 Origin: Human Host: Insect cells (Sf21) Recombinant SDS, Func Active
Casein Kinase 1, gamma 1 (CSNK1G1) (AA 293-393) protein (GST tag)CSNK1G1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Casein Kinase 1, gamma 1 (CSNK1G1) (AA 1-422) protein (Strep Tag)Crystallography grade CSNK1G1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Casein Kinase 1, gamma 1 (CSNK1G1) (AA 1-459) protein (Strep Tag)Crystallography grade CSNK1G1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- CSNK1G1 (Casein Kinase 1, gamma 1 (CSNK1G1))
- Alternative Name
- CSNK1G1/CKI-gamma 1 (CSNK1G1 Products)
- Synonyms
- CSNK1G1 Protein, csnk1g2 Protein, zgc:101563 Protein, CK1gamma1 Protein, 9130020E21Rik Protein, casein kinase 1 gamma 1 Protein, casein kinase 1, gamma 1 Protein, casein kinase 1 gamma 1 S homeolog Protein, CSNK1G1 Protein, csnk1g1 Protein, csnk1g1.S Protein, Csnk1g1 Protein
- Background
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Background: Casein kinase I isoform gamma-1, also known as CSNK1G1, is a member of the protein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I subfamily. The casein kinase I family of protein kinases are serine / threonine-selective enzymes that function as regulators of signal transduction pathways in most eukaryotic cell types. Casein has been used as a substrate since the earliest days of research on protein phosphorylation. Casein kinase activity associated with the endoplasmic reticulum of mammary glands was first characterized in 1974 and its activity was shown to not depend on cyclic AMP. The CKI family of monomeric serine-threonine protein kinases is found in eukaryotic organisms from yeast to human. Mammals have seven family members: alpha, beta 1, gamma 1, gamma 2, gamma 3, delta, and epsilon. The family members have the highest homology in their kinase domains (53%-98% identical) and differ from most other protein kinases by the presence of the sequence S-I-N instead of A-P-E in kinase domain VIII. The CKI family members appear to have similar substrate specificity and substrate selection is thought to be regulated via subcellular localization and docking sites in specific substrates.
Synonym: CK1gamma1
- Molecular Weight
- 76.3 kDa
- NCBI Accession
- NP_071331
- Pathways
- Hedgehog Signaling
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