CASP14 Protein (His tag)

Catalog No. ABIN7317468

Product Details

Target: CASP14 (Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CASP14 protein is labelled with His tag.

Purpose: Recombinant Human Caspase-14/CASP14 Protein (His Tag)(Active)

Sequence: Ser 2-Gln 242

Characteristics: A DNA sequence encoding the human CASP14 (NP_036246.1) (Ser 2-Gln 242) was expressed, with a polyhistide tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Biological Activity Comment: Measured by its ability to bind biotinylated Cynomolgus IL18 in a functional ELISA.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CASP14 (Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14))

Alternative Name: Caspase-14/CASP14 (CASP14 Products)

Synonyms: CASP14 Protein, MICE Protein, mini-ICE Protein, caspase 14, apoptosis-related cysteine peptidase Protein, caspase 14 Protein, caspase-14-like Protein, caspase-14 Protein, CASP14 Protein, Casp14 Protein, LOC485601 Protein, LOC780455 Protein

Background:

Background: Caspase 14 is a member of the caspase family. Caspases are a kind of cysteine proteinase consisting of a prodomain plus large and small catalytic subunits, that play a central role in cell apoptosis. Caspase 14 possesses an unusually short prodomain and is highly expressed in embryonic tissues but absent from most of the adult tissues except for the skin, which suggests a role in ontogenesis and skin physiology. Unlike the other short prodomain caspases(caspase-3, caspase-6, and caspase-7), Caspase 14 was not processed by multiple death stimuli including activation of members of the tumor necrosis factor receptor family and expression of proapaptotic members of the bcl-2 family. Caspase 14 has been described to be processed and activated by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may take part in keratinocyte terminal differentiation, which is essential for the skin barrier.

Synonym: Caspase-14, CASP-14, CASP14,MGC119078,MGC119079

Molecular Weight: 28.5 kDa

NCBI Accession: NP_036246