CHRDL2 Protein (His tag)
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- Target See all CHRDL2 Proteins
- CHRDL2 (Chordin-Like 2 (CHRDL2))
- Protein Type
- Recombinant
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Origin
- Mouse
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Source
- Human Cells
- Purification tag / Conjugate
- This CHRDL2 protein is labelled with His tag.
- Purpose
- Recombinant Mouse CHL2/CHRDL2 Protein (His Tag)
- Sequence
- Gln24-Leu426
- Characteristics
- Recombinant Mouse Chordin-like protein 2 is produced by our Mammalian expression system and the target gene encoding Gln24-Leu426 is expressed with a 6His tag at the C-terminus.
- Purity
- > 95 % as determined by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Chordin-Like 2 (CHRDL2) (AA 26-429) protein (His tag)
Crystallography grade CHRDL2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Chordin-Like 2 (CHRDL2) (AA 26-426) protein (His tag)Crystallography grade CHRDL2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Chordin-Like 2 (CHRDL2) protein (Myc-DYKDDDDK Tag)CHRDL2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- CHRDL2 (Chordin-Like 2 (CHRDL2))
- Alternative Name
- CHL2/CHRDL2 (CHRDL2 Products)
- Synonyms
- BNF1 Protein, CHL2 Protein, FKSG37 Protein, 1810022C01Rik Protein, Chl2 Protein, chl Protein, si:dkey-11j5.7 Protein, si:dkey-11j5.7-like Protein, chordin like 2 Protein, chordin-like 2 Protein, CHRDL2 Protein, Chrdl2 Protein, chrdl2 Protein
- Background
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Background: Mouse Chordin-Like 2, also known as CHL2, is a novel chordin family member with structural homology to CHL1 which is implicated in tumor angiogenesis. The mouse CHL2 gene encodes a 426 amino acids (aa) protein with a 25 aa signal peptide. The mature chain of CHL2 protein contains two potential N-linked glycosylation sites, one putative NLS and three 63 aa cysteine-rich von Willebrand type C repeats (CRs). CHL2 gene is weakly expressed in the liver and kidney, partly expressed in the connective tissues of reproductive organs such as ligaments of the ovary and oviduct in females, and of testis, epididymis and certain male accessory sex glands in males. Recombinant mCHL2 protein interacted directly with five BMPs and one GDF thereby inhibiting, in vitro, several BMP/GDF-dependent processes including, osteogenic differentiation of C2C12 mesenchymal progenitor cells by several BMPs, ATDC5 embryonal carcinoma cells by GDF5 and BMP4-dependent lymphohematopoietic (CD34+CD31hi and CD34+CD31lo) progenitor cell development from ES cells. CHL2 may inhibits BMPs activity by blocking their interaction with their receptors, and has a negative regulator effect on the cartilage formation/regeneration from immature mesenchymal cells, by preventing or reducing the rate of matrix accumulation. Also, it may play a role during myoblast and osteoblast differentiation, and maturation.
Synonym: Chordin-like protein 2, BNF-1, CHRDL2, chordin-like 2, chordin-like protein 2
- Molecular Weight
- 46.3 kDa
- UniProt
- Q8VEA6
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