F13B Protein (His tag)
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- Target See all F13B Proteins
- F13B (Coagulation Factor 13, B Polypeptide (F13B))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This F13B protein is labelled with His tag.
- Purpose
- Recombinant Human Coagulation Factor XIII B chain/F13B Protein (His Tag)
- Sequence
- Met 1-Thr 661
- Characteristics
- A DNA sequence encoding the human F13B (P05160) (Met 1-Thr 661) was expressed, fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product F13B Protein
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Coagulation Factor 13, B Polypeptide (F13B) (AA 21-661) protein (His tag)
Crystallography grade F13B Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Coagulation Factor 13, B Polypeptide (F13B) (AA 22-669) protein (His tag)Crystallography grade F13B Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- F13B (Coagulation Factor 13, B Polypeptide (F13B))
- Alternative Name
- Coagulation Factor XIII B chain/F13B (F13B Products)
- Synonyms
- F13B Protein, coagulation factor XIII B chain Protein, LOC100347263 Protein
- Background
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Background: Coagulation factor XIII B chain, also known as Fibrin-stabilizing factor B subunit, Protein-glutamine gamma-glutamyltransferase B chain, Transglutaminase B chain and F13B, is a secreted protein which contains 10 Sushi ( CCP / SCR ) domains. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as a plasma carrier molecules. Platelet factor XIII is composed of just 2 A subunits, which are identical to those of plasma origin. The B chain of factor XIII is not catalytically active, but is thought to stabilize the A subunits and regulate the rate of transglutaminase formation by thrombin. Factor XIII acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits, and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. Defects in F13B are the cause of factor XIII subunit B deficiency ( FA13BD ) which is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.
Synonym: Coagulation factor 13,Coagulation factor XIII,FXIIIB
- Molecular Weight
- 74.5 kDa
- UniProt
- P05160
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