CLPS Protein (His tag)

Catalog No. ABIN7321063

Product Details

Target: CLPS (Colipase, Pancreatic (CLPS))

Protein Type: Recombinant

Origin: Rat

Source: HEK-293 Cells

Purification tag / Conjugate: This CLPS protein is labelled with His tag.

Purpose: Recombinant Rat CLPS/Colipase Protein (His Tag)

Sequence: Met1-Gln112

Characteristics: A DNA sequence encoding the rat Clps (NP_037271.1) (Met1-Gln112) was expressed with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CLPS (Colipase, Pancreatic (CLPS))

Alternative Name: CLPS/Colipase (CLPS Products)

Synonyms: CLPS Protein, 2200003J09Rik Protein, COLQ Protein, colipase Protein, colipase, pancreatic Protein, CLPS Protein, Clps Protein

Background:

Background: Colipase belongs to the colipase family. Structural studies of the complex and of colipase alone have revealed the functionality of its architecture. It is a small protein with five conserved disulphide bonds. Structural analogies have been recognised between a developmental protein, the pancreatic lipase C-terminal domain, the N-terminal domains of lipoxygenases and the C-terminal domain of alpha-toxin. Colipase can only be detected in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Colipase allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase. Colipase is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilising as active conformation and considerably increasing the overall hydrophobic binding site.

Synonym: CLPS,Colipase

Molecular Weight: 11.9 kDa

NCBI Accession: NP_037271

Pathways: Lipid Metabolism