CSF1R Protein

Catalog No. ABIN7318000

This Recombinant CSF1R protein is expressed in HEK-293 Cells.

Quick Overview for CSF1R Protein (ABIN7318000)

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: ELISA, Cell Culture (CC)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human CSF1R/CD115 Protein

Sequence: Met 1-Glu 512

Characteristics: The mature form of human M-CSFR (NP_005202.2) extracellular domain (Met 1-Glu 512) with quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. Immobilized sMCSFR at 10 μg/ml (100 μl/well) can bind biotinylated human CSF1-His, The EC₅₀ of biotinylated human CSF1-His is 32-74. 8 ng/ml. 2. Measured by its ability to inhibit the human CSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is typically 0.3-1. 5 μg/mL in the presence of 3 ng/mL of rhM-CSF.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Alternative Name: CSF1R/CD115

Background: C-FMS;CD115;CSF-1 receptor;CSF-1-R;CSF-1R;CSF1R;FIM2;FMS;HDLS;M-CSF-R;MCSF Receptor ;Macrophage colony-stimulating factor 1 receptor;Proto-oncogene c-Fms,M-CSFR encoded by the proto-oncogene c-fms is the receptor for colony stimulating factor 1 (CSF1R), a cytokine involved in the proliferation, differentiation, and activation of macrophages. This cell surface glycoprotein is consisted by an extracellular ligand-binding domain, a single membrane-spanning segment, and an intracellular tyrosine kinase domain. Binding of CSF1 activates the receptor kinase, leading to "autophosphorylation" of receptor subunits and the concomitant phosphorylation of a series of cellular proteins on tyrosine residues. CSF1R is a tyrosine kinase receptor that is absolutely required for macrophage differentiation and thus occupies a central role in hematopoiesis. CSF1 and its receptor (CSF1R, product of c-fms proto-oncogene) were initially implicated as essential for normal monocyte development as well as for trophoblastic implantation. This apparent role for CSF1/CSF1R in normal mammary gland development is very intriguing because this receptor/ligand pair has also been found to be important in the biology of breast cancer in which abnormal expression of CSF1 and its receptor correlates with tumor cell invasiveness and adverse clinical prognosis. Tumor cell expression of CSF1R is under the control of several steroid hormones (glucocorticoids and progestins) and the binding of several bHLH transcription factors, while tumor cell expression of CSF-1 appears to be regulated by other hormones, some of which are involved in normal lactogenic differentiation.

Molecular Weight:

Calculated MW: 55.1 kDa

Observed MW: 80-90 kDa

Gene ID: 1436

NCBI Accession: NP_005202

UniProt: P07333

Pathways: RTK Signaling, Inositol Metabolic Process, Cell-Cell Junction Organization