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CLMP Protein (His tag)

CLMP Origin: Rat Host: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
Catalog No. ABIN7321189
  • Target See all CLMP Proteins
    CLMP (CXADR-Like Membrane Protein (CLMP))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Origin
    • 9
    • 4
    • 1
    Rat
    Source
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This CLMP protein is labelled with His tag.
    Purpose
    Recombinant Rat ASAM Protein (His Tag)(Active)
    Sequence
    Met1-Met232
    Characteristics
    A DNA sequence encoding the rat ASAM (Q8K1G0) (Met1-Met232) was expressed, fused with a polyhistidine tag at the C-terminus.
    Purity
    > 85 % as determined by SDS-PAGE
    Endotoxin Level
    < 1.0 EU per μg of the protein as determined by the LAL method
    Biological Activity Comment
    Measured by the ability of the immobilized protein to support the adhesion of MS-1 cells. When 5 x 10E4 cells/well are added to Recombinant Rat ASAM coated plates (12.5 μg/mL with 100 μL/well), 35-70% cells will adhere after 1 hour incubation at 37°C.
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  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Please refer to the printed manual for detailed information.
    Buffer
    Lyophilized from sterile PBS, pH 7.4
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • Target
    CLMP (CXADR-Like Membrane Protein (CLMP))
    Alternative Name
    ASAM (CLMP Products)
    Synonyms
    ACAM Protein, ASAM Protein, CSBM Protein, CSBS Protein, Asam Protein, Ol16 Protein, 9030425E11Rik Protein, ASP5 Protein, AW557819 Protein, CXADR like membrane protein Protein, CXADR-like membrane protein Protein, CLMP Protein, Clmp Protein
    Background

    Background: Adipocyte-specific adhesion molecule (ASAM), also known as ACAM and CLMP, is a type I transmembrane protein and a member of the CTX (cortical thymocyte marker in Xenopus) family within the immunoglobulin superfamily. ASAM protein is highly expressed in the small intestine and placenta, and is found at intermediate levels in the heart, skeletal muscle, colon, spleen, kidney, and lung, and appears in low levels in the liver and peripheral blood leukocytes as well. ASAM is a transmembrane component of tight junctions in epithelial cells that can mediate cell aggregation and regulate transepithelial resistance across polarized epithelial cells. In addition, its expression is strongly correlated with white adipose tissue (WAT) mass of human and rodents with obesity.

    Synonym: ASAM,Clmp ,Acam

    Molecular Weight
    25.6 kDa
    UniProt
    Q8K1G0
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